Boosting of enzymatic softwood saccharification by fungal GH5 and GH26 endomannanases
نویسندگان
چکیده
منابع مشابه
Molecular Engineering of Fungal GH5 and GH26 Beta-(1,4)-Mannanases toward Improvement of Enzyme Activity
Microbial mannanases are biotechnologically important enzymes since they target the hydrolysis of hemicellulosic polysaccharides of softwood biomass into simple molecules like manno-oligosaccharides and mannose. In this study, we have implemented a strategy of molecular engineering in the yeast Yarrowia lipolytica to improve the specific activity of two fungal endo-mannanases, PaMan5A and PaMan...
متن کاملNMR analysis of the binding mode of two fungal endo-β-1,4-mannanases from GH5 and GH26 families.
The enzymatic digestion of the main components of lignocellulosic biomass, including plant cell wall mannans, constitutes a fundamental step in the renewable biofuel production, with great potential benefit in the industrial field. Despite several reports of X-ray structures of glycoside hydrolases, how polysaccharides are specifically recognized and accommodated in the enzymes binding site sti...
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Sweet sorghum bagasse (SSB) from food processing and agricultural industry has attracted the attention for uses in production of biofuel, enzymes and other products. The alteration in lignocellulolytic enzymes by use of supplements in fungal pretreatment of SSB to achieve higher lignin degradation, selectivity value and enzymatic hydrolysis to fermentable sugar was studied. Fungal strain Coriol...
متن کاملEnhancement of Enzymatic Saccharification of Poplar by Green Liquor Pretreatment
Green liquor (Na2S + Na2CO3, GL) pretreatment is an effective pathway for improving the enzymatic digestibility of lignocellulosic biomass for the production of bioethanol. In this work, GL was employed as a pretreatment to enhance the enzymatic saccharification of poplar. During pretreatment, the increase of H-factor and TTA charge resulted in enhanced delignification and increased degradation...
متن کاملContinuous enzymatic liquefaction of starch for saccharification.
A process was explored for continuous enzymatic liquefaction of corn starch at high concentration and subsequently saccharification to glucose. The process appears to be quite efficient for conversion of starch to glucose and enzymatic liquefaction and should be readily adaptable to industrial fermentation processes. Preliminary work indicated that milled corn or other cereal grains also can be...
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ژورنال
عنوان ژورنال: Biotechnology for Biofuels
سال: 2018
ISSN: 1754-6834
DOI: 10.1186/s13068-018-1184-y